产品货号：
GS2136
中文名称：
BstKTI内切酶
英文名称：
BstKTI
产品规格：
100U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：BstKTI内切酶，BstKTI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GAT↑C |
| 来源 | Bacillus stearothermophilus KT |
| Assayed on | Lambda DNA(dam-) |
| Optimal buffer | W?(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;100 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Blocked by overlapping Dam methylation(GmATC)?GATC.|Not blocked by CG methylation.|Cut hemimethylated site:5- GmATC-3/5-GATC-3 |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BfuCI^,BscFI^,Bsp143I^,BssMI^,BstENII^,BstMBI^,DpnI^,Kzo9I^,MalI^,MboI^,NdeII^,Sau3AI^ |
相关搜索：BstKTI内切酶，BstKTI限制性内切酶